The primary cell line panel was derived from EAC cases included in the ICGC sequencing study, including MFD (Tim Underwood, Southampton, OCCAMS consortium member), OES127 (Anna Grabowska, Nottingham, OCCAMS consortium member) and CAM02 (organoid, Mathew Garnett, Cambridge). The MFD line required 10% fetal calf serum (PAA) in DMEM medium (Invitrogen, ThermoFisher Scientific) and the CAM02 culture method was as previously described [51 (link)]. The feeder layer system was used to expand OES127 lines. The established EAC lines, SK-GT-4, OAC-P4C, OACM5.1C, and OE33 were cultured in RPMI medium (Sigma) with 10% fetal calf serum, except for FLO-1, which was grown in DMEM with 10% fetal calf serum. The identity of all cell lines was verified by short tandem repeat (STR) profiling and routinely examined for mycoplasma contamination.
Small molecular inhibitors used for treatment were: Lapatinib, AZD-4547, Olaparib, MK-1775 and AZD-7762 (BioVision), Crizotinib (LKT Labs) and Topotecan (Cayman Chemical). Inhibitors were diluted to working concentrations in DMSO (Sigma).
Small molecular inhibitors used for treatment were: Lapatinib, AZD-4547, Olaparib, MK-1775 and AZD-7762 (BioVision), Crizotinib (LKT Labs) and Topotecan (Cayman Chemical). Inhibitors were diluted to working concentrations in DMSO (Sigma).
