fHb, as hemolysis marker for RBC, was measured with a colorimetric assay kit (Catachem Inc., Oxford, England). fHb determination method was based upon the peroxidase activity of hemoglobin. Hemoglobin activates the oxidation of 3.3’, 5.5’-tetramethylbenzidine by hydrogen peroxide to form a chromogenic product with maximum absorption at 650 nm (20 (link)).
Myeloperoxidase (MPO), as hemolysis marker for WBC, was measured with an enzyme immunoassay test kit (Biocheck Inc., Foster City, California, USA). And finally, ᵝ-thromboglobulin (ᵝ-TG), as hemolysis marker for PLT, was measured with an enzyme immunoassay test kit (CUSABIO Inc., Wuhan, China) (21 (link), 22 (link)).
Myeloperoxidase (MPO), as hemolysis marker for WBC, was measured with an enzyme immunoassay test kit (Biocheck Inc., Foster City, California, USA). And finally, ᵝ-thromboglobulin (ᵝ-TG), as hemolysis marker for PLT, was measured with an enzyme immunoassay test kit (CUSABIO Inc., Wuhan, China) (21 (link), 22 (link)).
