Theanine Extraction and Analysis

The hot water was employed to extract theanine from various tissues of tea leaves, described by the previous method [33 (link)]. To investigate the involvement of CsGGT4 in the production and degradation of theanine, samples were collected and examined utilizing HPLC–MS/MS and GC/MS techniques, following established protocols in a previous study [19 (link)]. The instrument parameters and methods employed were in accordance with Dong et al. [61 (link)].

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Chang M., Sun Y., Fang K., Fu M., Ma J., Gao Y., Chen Q., Liu L., Zhang Z., Wan X, & Sun J. (2024). CsMYB73 negatively regulates theanine accumulation mediated by CsGGT2 and CsGGT4 in tea shoots (Camellia sinensis). Horticulture Research, 11(3), uhae012.

Publication 2024

Corresponding Organization :

Other organizations : Anhui Agricultural University

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Variable analysis

independent variables

Extraction of theanine from various tissues of tea leaves using hot water

dependent variables

Production and degradation of theanine
Theanine levels measured using HPLC–MS/MS and GC/MS techniques

control variables

Instrument parameters and methods employed were in accordance with previous study [61 (link)]

controls

Positive and negative controls not explicitly mentioned

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