Aqueous solutions of 0.2 mM H2O2, 1 μM flg22 aqueous solution, and 0.1 mg/mL chitin solution were mixed with 0.03% v/v Silwet L-77. Each solution was individually sprayed on the top of 30-day-old plants. The top one-third of fully expanded leaves on the plants were used for H2O2 transport, callose deposition, and qRT-PCR.
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