Comprehensive Western Blotting Analysis

Western blotting analysis was performed as described [32 (link)]. Antibodies used were anti-CAV1 rabbit antibody, anti-Flag rabbit antibody, anti-p-STAT3 rabbit antibody, anti-Acetyl-Stat3 (Lys685) rabbit antibody, anti-STAT3 mouse antibody, anti-p-AKT rabbit antibody, and anti-AKT rabbit antibody from Cell Signaling Technologies (Beijing, China), anti-α-Tubulin mouse antibody, and anti-GAPDH mouse antibody, from Santa Cruz Biotechnology (Dallas, TX, USA). Anti-DNMT1 mouse antibody was from Abcam (Cambridge, MA, USA). Anti-His mouse antibody was obtained from Beyotime Institute of Biotechnology (Nantong, Jiangsu, China). Anti-vIL-6 rabbit monoclonal antibody was kindly provided by Dr Robert Yarchoan from Center for Cancer Research, National Cancer Institute (Bethesda, Maryland, USA).

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Li W., Wang Q., Qi X., Guo Y., Lu H., Chen Y., Lu Z., Yan Q., Zhu X., Jung J.U., Tosato G., Gao S.J, & Lu C. (2020). Viral interleukin-6 encoded by an oncogenic virus promotes angiogenesis and cellular transformation by enhancing STAT3-mediated epigenetic silencing of caveolin 1. Oncogene, 39(23), 4603-4618.

Publication 2020

anti-Flag rabbit antibody anti-p-STAT3 rabbit antibody anti-STAT3 mouse antibody anti-AKT rabbit antibody anti-α-Tubulin mouse antibody anti-GAPDH mouse antibody

Anti antibody Antibodies Antibody Cancer Cav1 Dnmt1 Gapdh Mouse Rabbit Stat3 Western blotting analysis α tubulin

Corresponding Organization : Nanjing University of Chinese Medicine

Other organizations : Nanjing Medical University, Jiangsu Province Hospital, University of Southern California, National Cancer Institute, Center for Cancer Research, UPMC Hillman Cancer Center

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Variable analysis

independent variables

Treatment with anti-CAV1 rabbit antibody
Treatment with anti-Flag rabbit antibody
Treatment with anti-p-STAT3 rabbit antibody
Treatment with anti-Acetyl-Stat3 (Lys685) rabbit antibody
Treatment with anti-STAT3 mouse antibody
Treatment with anti-p-AKT rabbit antibody
Treatment with anti-AKT rabbit antibody
Treatment with anti-α-Tubulin mouse antibody
Treatment with anti-GAPDH mouse antibody
Treatment with anti-DNMT1 mouse antibody
Treatment with anti-His mouse antibody
Treatment with anti-vIL-6 rabbit monoclonal antibody

dependent variables

Protein expression levels measured by Western blotting analysis

control variables

Western blotting protocol as described in the referenced publication [32]

controls

Positive controls: Not explicitly mentioned
Negative controls: Not explicitly mentioned

Annotations

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