Nanoliposome Characterization by DLS

The mean hydrodynamic diameter and polydispersity index (PDI) of nanoliposomes were examined through the Malvern Zetasizer Nano ZS device (Malvern Instruments, Malvern, UK) in dynamic light scattering (DLS) mode. First, the samples were mixed with ultrapure water at a ratio of 1:400. Next, they were placed inside vertically cylindrical covets with a diameter of 10 mm. Measurements were performed at the scattering angle of 173° and temperature of 25 °C. Further, the refractive index and extent of absorbance for the samples were considered as 1.471 and 0.01, respectively [29 (link)]. For all of the samples, the measurements were performed with three replications, as the results were reported as mean value ± SD.

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Homayoonfal M., Mousavi M., Kiani H., Askari G., Desobry S, & Arab-Tehrany E. (2022). Modifying the Stability and Surface Characteristic of Anthocyanin Compounds Incorporated in the Nanoliposome by Chitosan Biopolymer. Pharmaceutics, 14(8), 1622.

Publication 2022

Zetasizer Nano ZS device

Device Hydrodynamic Replications

Corresponding Organization :

Other organizations : Biofuel Research Team, Université de Lorraine

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Variable analysis

independent variables

None mentioned

dependent variables

Mean hydrodynamic diameter of nanoliposomes
Polydispersity index (PDI) of nanoliposomes

control variables

Scattering angle of 173°
Temperature of 25 °C
Refractive index of 1.471
Absorbance extent of 0.01

controls

Positive control: None mentioned
Negative control: None mentioned

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