Extraction and Analysis of Cell Wall Phenolics
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Corresponding Organization : Joint BioEnergy Institute
Other organizations : Kyoto University, Appalachian State University
Variable analysis
- Mild acidolysis treatment duration (up to 4 h)
- Phenolic compounds detected and analyzed via UHPLC-MS
- Amount of CWRs (10 mg)
- Concentration of TFA (50 mM)
- Incubation temperature (100 °C)
- Shaking speed (750 rpm)
- Centrifugation speed (19,500 g)
- Methanol concentration (80%) for re-dissolving dried supernatant
- NaOH concentration (2 N) for alkaline digestion
- HCl concentration (6 N) for neutralization
- Internal standard (t-o-coumaric acid) for wall-bound phenolics analysis
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