Assessing HuNoV Infectivity with HIE Assay

HuNoV infectivity was assessed via in vitro HIE assay as previously described.^{25 (link),45 (link)} Importantly, this method uses RT-qPCR to assess viral replication
in HIE cells by comparing the increase of norovirus genomic copies
at 72 h postinfection with the genomic copies at 1 h postinfection
(no amplification). Additional details are provided in the SI.

Partial Protocol Preview
This section provides a glimpse into the protocol.
The remaining content is hidden due to licensing restrictions, but the full text is available at the following link: Access Free Full Text.

Kennedy L.C., Costantini V.P., Huynh K.A., Loeb S.K., Jennings W.C., Lowry S., Mattioli M.C., Vinjé J, & Boehm A.B. (2023). Persistence of Human Norovirus (GII) in Surface Water: Decay Rate Constants and Inactivation Mechanisms. Environmental Science & Technology, 57(9), 3671-3679.

Publication 2023

Assay Cells Genomic Norovirus

Corresponding Organization : Stanford University

Other organizations : Centers for Disease Control and Prevention, McGill University

Top 5 similar protocols

Variable analysis

independent variables

None explicitly mentioned

dependent variables

Increase of norovirus genomic copies at 72 h postinfection compared to 1 h postinfection (no amplification)

control variables

None explicitly mentioned

controls

Positive control: Not specified
Negative control: Not specified

Annotations

Based on most similar protocols

Etiam vel ipsum. Morbi facilisis vestibulum nisl. Praesent cursus laoreet felis. Integer adipiscing pretium orci. Nulla facilisi. Quisque posuere bibendum purus. Nulla quam mauris, cursus eget, convallis ac, molestie non, enim. Aliquam congue. Quisque sagittis nonummy sapien. Proin molestie sem vitae urna. Maecenas lorem.

As authors may omit details in methods from publication, our AI will look for missing critical information across the 5 most similar protocols.

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!