Selenium Modulates MAPK Pathway Activation

Western blotting was used to determine the effect of selenium on phosphorylation (i.e., activation) of the MAPK signalling pathway protein ERK. Western blotting was performed as previously described [52 (link)]. Mouse monoclonal pERK (sc-7383; Santa Cruz Biotechnology) and rabbit polyclonal ERK (sc-93; Santa Cruz Biotechnology, Dalla, Texas, USA) were used at 0.2 μg/mL. Membranes were viewed and analysed using the ChemiDoc MP system (Bio-Rad Laboratories; Gladesville, NSW, Australia). Semi-quantitative analysis of the relative density of the bands in Western blots was performed using Quantity One 4.2.1 image analysis software (Bio-Rad Laboratories, Hercules, CA, USA).

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Nguyen-Ngo C., Perkins A.V, & Lappas M. (2022). Selenium Prevents Inflammation in Human Placenta and Adipose Tissue In Vitro: Implications for Metabolic Diseases of Pregnancy Associated with Inflammation. Nutrients, 14(16), 3286.

Publication 2022

sc-7383; sc-93 ChemiDoc MP system Quantity One 4.2.1 image analysis software

Mapk signalling pathway Membranes Mouse Phosphorylation Protein Rabbit Selenium Western blots

Corresponding Organization : Griffith University

Other organizations : University of Melbourne

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Variable analysis

independent variables

Selenium

dependent variables

Phosphorylation (activation) of the MAPK signaling pathway protein ERK

control variables

Experimental conditions as previously described in reference [52]

controls

Positive control: Not specified
Negative control: Not specified

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