Human iPSC Culture and Maintenance

Human BC1, an iPSC line which was obtained from Dr. Linzhao Cheng’s lab at Johns Hopkins School of Medicine, was used in the study. This is an established cell line with published results [58 (link)]. Cells were cultured in StemFlex media (Gibco) on 6-well tissue culture plates coated with laminin (Biolamina). Cells were dissociated with StemPro Accutase (Gibco) into single-cell suspension and seeded in required density for the experiment. The ROCK inhibitor Y-27632 dihydrochloride (Tocris) was added on the first day of passage at a concentration of 10 µM. Cultured cells were tested to ensure they lack mycoplasma contamination.

Free full text: Click here

Das D., Sonthalia S., Stein-O.’Brien G., Wahbeh M., Feuer K., Goff L., Colantuoni C., Mahairaki V, & Avramopoulos D. (2024). Insights for disease modeling from single-cell transcriptomics of iPSC-derived Ngn2-induced neurons and astrocytes across differentiation time and co-culture. BMC Biology, 22, 75.

Publication 2024

StemFlex media laminin StemPro Accutase ROCK inhibitor Y-27632 dihydrochloride

Corresponding Organization : Johns Hopkins University

Top 5 similar protocols

Variable analysis

independent variables

Passage of cells using StemPro Accutase into single-cell suspension
Seeding of cells at required density for the experiment
Addition of ROCK inhibitor Y-27632 dihydrochloride at a concentration of 10 µM on the first day of passage

dependent variables

Measured outcomes from the experiment

control variables

Culturing of cells in StemFlex media (Gibco) on 6-well tissue culture plates coated with laminin (Biolamina)
Lack of mycoplasma contamination in cultured cells

controls

Positive control: Not specified
Negative control: Not specified

Annotations

Based on most similar protocols

Etiam vel ipsum. Morbi facilisis vestibulum nisl. Praesent cursus laoreet felis. Integer adipiscing pretium orci. Nulla facilisi. Quisque posuere bibendum purus. Nulla quam mauris, cursus eget, convallis ac, molestie non, enim. Aliquam congue. Quisque sagittis nonummy sapien. Proin molestie sem vitae urna. Maecenas lorem.

As authors may omit details in methods from publication, our AI will look for missing critical information across the 5 most similar protocols.

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!