Heart-Infiltrating Cell Isolation

The heart-infiltrating cells were isolated, as previously described (18 (link)). Briefly, the heart tissues were obtained and cut into pieces after which the tissue fragments were digested with 1 mg/mL of collagenase B (Roche 11088815001) or Collagenase Type 2 (Sangon Biotech A004174-0100) in HEPES buffer then rotated gently for 45 min at 37°C. In addition, cell suspensions were filtered with 70-μm cell strainers (BIOFIL) and Percoll (Beijing Solarbio Science & Technology Co., Ltd.) was used to purify heart mononuclear cells through density centrifugation.

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Xu H., Yu J., Cui J., Chen Z., Zhang X., Zou Y., Du Y., Li Y., Le S., Jiang L., Xia J, & Wu J. (2021). Ablation of Survivin in T Cells Attenuates Acute Allograft Rejection after Murine Heterotopic Heart Transplantation by Inducing Apoptosis. Frontiers in Immunology, 12, 710904.

Publication 2021

collagenase B Collagenase Type 2 70-μm cell strainers Percoll

Buffer Cell Centrifugation Collagenase 1 Collagenase 2 Heart Hepes Percoll Tissue

Corresponding Organization : Huazhong University of Science and Technology

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Variable analysis

independent variables

Collagenase B (Roche 11088815001)
Collagenase Type 2 (Sangon Biotech A004174-0100)

dependent variables

Heart-infiltrating cells

control variables

HEPES buffer
Density centrifugation using Percoll

controls

Positive control: Not specified
Negative control: Not specified

Annotations

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