The intracellular cAMP concentration was measured according to the previous method with some modifications [15 (link)]. YEM30 strain treated with different doses of MPD was incubated in RPMI 1640 medium at 37°C. After 12 h incubation, the intracellular cAMP concentration was measured by a cAMP ELISA Kit (Elabscience Biotechnology Co., China).
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Intracellular cAMP Concentration Measurement
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Elisa
Intracellular
Strain
Corresponding Organization : Xuzhou Medical College
Other organizations : Xuzhou Central Hospital, Xuchang University
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Variable analysis
independent variables
- Different doses of MPD
- Intracellular cAMP concentration
- YEM30 strain
- RPMI 1640 medium
- 37°C incubation temperature
- 12 h incubation duration
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