Metabolic and Microbial Community Analysis

The metabolic data were analyzed by one-way analysis of variance (ANOVA) in SPSS 21.0 software (SPSS Inc., Chicago, IL). When sampling time was included, data were analyzed using a linear mixed model with treatment and treatment by sampling time interaction as fixed effects, and sampling time as a repeated measures variable. The microbial community composition data was analyzed using Wilcoxon rank-sum test in the JMP Pro software (JMP Pro version 13.2.1, SAS Institute Inc. SAS Institute, Cary, NC, USA). The genomic ranks of attributes were evaluated by Correlation, ReliefF, Symmetrical Uncert, and multi-cluster feature selection (MCFS) methods in software of Waikato Environment for Knowledge Analysis (WEKA) (version 3.8.4, Hamilton, New Zealand) [32 ], and further comprehensively analyzed by RobustRankAggreg (RRA) R package [33 (link)]. All p values were adjusted for False Discovery Rate (FDR) using the Benjamini-Hochberg method. Statistical significance was declared at p ≤ 0.05 and tendencies at 0.05 < p ≤ 0.10.

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Li Q.S., Wang R., Ma Z.Y., Zhang X.M., Jiao J.Z., Zhang Z.G., Ungerfeld E.M., Yi K.L., Zhang B.Z., Long L., Long Y., Tao Y., Huang T., Greening C., Tan Z.L, & Wang M. (2022). Dietary selection of metabolically distinct microorganisms drives hydrogen metabolism in ruminants. The ISME Journal, 16(11), 2535-2546.

Publication 2022

JMP Pro

Genomic Microbial community composition Sampling treatment

Corresponding Organization :

Other organizations : Institute of Subtropical Agriculture, Chinese Academy of Sciences, University of Chinese Academy of Sciences, Lanzhou University, Yunnan University, Instituto de Investigaciones Agropecuarias, Hunan Academy of Agricultural Sciences, Shanghai Institute of Nutrition and Health, Australian Regenerative Medicine Institute, Monash University

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Variable analysis

independent variables

Treatment

dependent variables

Metabolic data
Microbial community composition

control variables

Sampling time

controls

No positive or negative controls were explicitly mentioned.

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