Transgenic Mouse Lines for Stem Cell Research

The Lgr5-EGFP-IRES-CreERT2, Hopx-CreERT2, Hopx-GFP, Rosa-LSL-DTR and Rosa-LSL-tdTomato mice were purchased from Jackson Laboratory. The Axin2-CreERT2-tdTomato line was a kind gift from Dr. Edward E. Morrisey (University of Pennsylvania). Male mice 8-12 weeks of age were used to perform experiments. Detailed information on generation and genotyping of those mouse lines has been published previously (Barker et al., 2007 (link), Buch et al., 2005 (link), Frank et al., 2016 (link), Takeda et al., 2011 (link), Takeda et al., 2013 (link)). All animal work was performed in accordance to the protocols approved by the Washington University School of Medicine Animal Studies Committee.