Glycoprotein Expression Plasmid Construction

Codon-optimized CVS-G and SAD-G were commercially synthesized (Invitrogen) and cloned into pcDNA3.1. Mutations were introduced via Q5 site-directed mutagenesis (New England Biolabs) and constructs were cloned into pCAGGS (Addgene). pCAGGS-CVS-G accession number is ABV24348.1 and pCAGGS-SAD-G accession number is P16288.1. pCAGGS-DOG-G (accession number CUI02214.1) and EBLV1-G (accession number SMD54588.1) have been described previously [16, 34 (link)]. pCAGGS-CVS_SADT and pCAGGS-EBLV_SADT, comprising the SAD cytoplasmic tail sequence, were generated through PCR mutagenesis.

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Almasoud I., Charlton F.W., Finke S., Barr J.N, & Mankouri J. (2023). Internalization of rabies virus glycoprotein differs between pathogenic and attenuated virus strains. The Journal of General Virology, 104(12), 001935.

Publication 2023

Q5 site-directed mutagenesis pCAGGS

Corresponding Organization :

Other organizations : University of Leeds, Kuwait University, Friedrich-Loeffler-Institut

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Variable analysis

independent variables

Codon-optimized CVS-G
Codon-optimized SAD-G
Mutations introduced via Q5 site-directed mutagenesis
PCAGGS-CVS_SADT
PCAGGS-EBLV_SADT

dependent variables

Not explicitly mentioned

control variables

PcDNA3.1
PCAGGS
PCAGGS-DOG-G
PCAGGS-EBLV1-G

positive controls

Not specified

negative controls

Not specified

Annotations

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