VEEV TC-83 Virus Propagation in BHK Cells

Vero 76 cells (ATCC CRL-1587) were maintained at 37°C and 5% CO₂ in complete medium containing Dulbecco’s modified Eagle medium with Hi-glucose and L-glutamine supplemented with 10% fetal bovine serum (FBS) and 1% penicillin-streptomycin. VEEV TC-83 was obtained from Connie Schmaljohn (United States Army Medical Research Institute of Infectious Diseases, Maryland). Virus seed stocks were amplified in Bovine hamster kidney (BHK)cells in minimum essential medium with Earle’s salts Eagle's minimum essential medium (EMEM) with 2% FBS and 1% penicillin-streptomycin. All cell culture reagents were purchased from Thermo Fisher Scientific unless otherwise specified. VEEV TC-83 was sequenced as a reference genome as previously reported (34 (link)).