S536 and S1367 cells were transformed with APs and CPs as indicated in Supplementary table 2 . Freshly saturated cultures of single colonies grown in Davis Rich Media19 (link) (DRM) plus maintenance antibiotics were diluted 500-fold into DRM media with maintenance antibiotics in a 96-well deep-well plate (Axygen) and induced with indicated concentrations of arabinose (Gold Biotechnology) before incubation for 2 h at 37 °C with shaking at 230 RPM. 150 μL of cells per well were then transferred to a 96-well black-walled clear-bottomed plate with a transparent lid (Costar). 600 nm absorbance and luminescence were read at 15-min intervals over an 8-h kinetic cycle with shaking at 230 RPM between reads using a Tecan Spark multimode microplate reader (Tecan). Single read data were taken at peak luminescence value (4–5 h post-induction). OD600-normalized luminescence values were determined by dividing raw luminescence by background-subtracted (DRM only) 600 nm absorbance.
For phage-induced luciferase timecourse assay, S536 and S2060 cells were transformed with APs and diluted in DRM as described above. Cells were grown to an OD600 of 0.4 and were inoculated with selection phage at an initial titer of 5 × 104 pfu/mL. 150 μL of cells per well were immediately transferred to a plate for luminescence and optical density reading in a kinetic cycle as described above.
For phage-induced luciferase timecourse assay, S536 and S2060 cells were transformed with APs and diluted in DRM as described above. Cells were grown to an OD600 of 0.4 and were inoculated with selection phage at an initial titer of 5 × 104 pfu/mL. 150 μL of cells per well were immediately transferred to a plate for luminescence and optical density reading in a kinetic cycle as described above.
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