As reported by a previous study (31 (link)), we also used the Magna RIP RNA-Binding Protein Immunoprecipitation kit (Millipore, Billerica, USA) to conduct the anti-AGO2 RIP assay. Extracts of the treated SK-MES-1R and NCI-H226R cells in RIP buffer were incubated with normal rabbit IgG (Proteintech Group, Inc.; Wuhan, China) and AGO2 antibodies (Cell Signaling Technology), which were combined with magnetic beads. We isolated the immunoprecipitated RNAs and examined genes using RT-qPCR.
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Anti-AGO2 RIP Assay Protocol
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Corresponding Organization : Hebei Medical University
Other organizations : Jilin University, First Hospital of Jilin University
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Variable analysis
independent variables
- Treatment of SK-MES-1R and NCI-H226R cells
- Genes examined using RT-qPCR
- Normal rabbit IgG (Proteintech Group, Inc.; Wuhan, China)
- Positive control: AGO2 antibodies (Cell Signaling Technology)
- Negative control: Normal rabbit IgG (Proteintech Group, Inc.; Wuhan, China)
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