Identifying miR-145-3p Regulated Genes in mCRPC

We performed a combination of in silico and genome-wide gene expression analyses. First, genes regulated by miR-145-3p were listed using the TargetScan database (release 7.0). Next, we performed genome-wide gene expression analysis using miR-145-3p-transfected PC3 cells. A SurePrint G3 Human GE 60 K Microarray (Agilent Technologies) was used for expression profiling of miRNA transfectants in comparison with mock PC3 cells(accession number: GSE77790, log₂ ratio<−1.5). Next, we identified genes that were highly upregulated in mCRPC tissue compared with those in non-PCa and HSPC and highly upregulated in PCa compared with those in non-PCa tissues (accession number: GSE35988; fold change > 2 and q-value<0.05). Finally, to validate that these genes were upregulated in mCRPC clinical specimens, we used other data sets from cBioportal (http://www.cbioportal.org/) and GEO database (GSE 70770) (Ross-Adams et al, 2015 (link); Whitington et al, 2016 (link)). HSPC data were obtained from TCGA-PRAD (Cancer Genome Atlas Research N, 2015 (link)), and mCRPC data were obtained from SU2c/PCF (Robinson et al, 2015 (link)).