Multiplex Cytokine Analysis in Pericardial Fluid

Magnetic Luminex^® assays are magnetic bead-based antibody microarrays that can be used to facilitate simultaneous quantitation in a single sample [17 (link)]. Previous studies have demonstrated the feasibility of using Luminex to measure cytokines in pericardial fluid [16 (link),18 (link),19 (link)]. In the study, we custom-made a Luminex (R&D Systems, Inc., Minneapolis, MN, USA) panel including MPO to assess the impact of pericardial fluid on POAF. In addition, all the pericardial fluid samples were optimally diluted to ensure cytokine levels fell within the dynamic detection range of the assay. According to the manufacturer’s instructions, all the standards and samples were run in duplicate.

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Liu Y., Yang Y., Yang X, & Hua K. (2022). Myeloperoxidase Levels in Pericardial Fluid Is Independently Associated with Postoperative Atrial Fibrillation after Isolated Coronary Artery Bypass Surgery. Journal of Clinical Medicine, 11(23), 7018.

Publication 2022

Luminex

Antibody Assay Cytokine Microarrays Pericardial fluid

Corresponding Organization : Capital Medical University

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Variable analysis

dependent variables

Cytokine levels in pericardial fluid

control variables

Pericardial fluid samples were optimally diluted to ensure cytokine levels fell within the dynamic detection range of the assay
All the standards and samples were run in duplicate according to the manufacturer's instructions

controls

Positive control: Not specified
Negative control: Not specified

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