RNA Extraction and qRT-PCR Analysis

RNA samples were extracted with Trizol reagent (Sigma-Aldrich, St. Louis, MO, USA). A cDNA synthesis was performed with the HiScript II Q RT kit (Vazyme Biotech, Nanjing, China). The quantitative real-time PCR (qRT-PCR) analysis was performed as previously described [32 (link)]. Primers were synthesized by Sangon Biotech (Shanghai, China). The primer sequences are listed in Table S2.

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Zhang Z., Du J., Xu Q., Li Y., Zhou S., Zhao Z., Mu Y., Zhao A.Z., Cao S.M, & Li F. (2022). Resistin Promotes Nasopharyngeal Carcinoma Metastasis through TLR4-Mediated Activation of p38 MAPK/NF-κB Signaling Pathway. Cancers, 14(23), 6003.

Publication 2022

Trizol reagent HiScript II Q RT kit

Cdna Primer Quantitative real time pcr analysis Synthesis Trizol

Corresponding Organization : Sun Yat-sen University Cancer Center

Other organizations : Guangdong Medical College

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Variable analysis

independent variables

No independent variables explicitly mentioned

dependent variables

Gene expression levels measured by quantitative real-time PCR (qRT-PCR)

control variables

RNA samples extracted with Trizol reagent (Sigma-Aldrich, St. Louis, MO, USA)
CDNA synthesis performed with the HiScript II Q RT kit (Vazyme Biotech, Nanjing, China)
Primers synthesized by Sangon Biotech (Shanghai, China)

controls

No positive or negative controls specified

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