We used an ELISA kit to measure circulating GPHB5 levels according to the manufacturer’s instructions (Kete Biotechnology Co., Ltd, Jiangshu, China). This kit has high sensitivity and specificity for the measurement of GPHB5 without obvious cross-reactivity. The intra- and interassay coefficients of variation (CVs) were< 10% and< 8%, respectively. As previously published (23 (link)), serum adiponectin (Adipoq) concentrations were measured by an ELISA (sk00010-01, Aviscerabioscience Inc., USA), with intra- and inter-assay CVs of 4% - 8% and 8% - 12%, respectively.
Wang Y., Xiang T., Xia X., Zhang H., Geng S., Yang G., Qiu S., He Y., Liu R., Li L., Liu H., Li K., Zhang L., Liang Z, & He J. (2022). Elevated circulating GPHB5 levels in women with insulin resistance and polycystic ovary syndrome: A cross-sectional study and multiple intervention studies. Frontiers in Endocrinology, 13, 1010714.
Other organizations :
Chongqing Medical University, Second Affiliated Hospital of Chongqing Medical University, Dalian Medical University, First People's Hospital of Chongqing, Chongqing Dazu District People's Hospital, University of Mississippi Medical Center
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