Humanized Mouse Models for HIV Research

Mice were created and monitored as previously described (7 (link), 12 (link), 13 (link)). BLT mice were generated by implanting human thymus and liver tissue under the kidney capsule of sub-lethally irradiated NOD.Cg-Prkdcscid Il2rgtm1Wjl/SzJ (NSG, The Jackson Laboratory) mice. BLT mice received 3.5×10⁵ autologous CD34+ hematopoietic stem cells. Humanized MoM were created by transplanting sub-lethally irradiated NOD.CB17-Prkdcscid/J mice (NOD/SCID, The Jackson Laboratory) with approximately 1×10⁶ cord blood or liver-derived CD34+ hematopoietic stem cells. All animals were irradiated prior to stem cell transplantation (BLT mice: 200 rad, MoM: 250 rad). Mice were maintained in a specific pathogen-free facility by the Division of Laboratory Animal Medicine at the University of North Carolina at Chapel Hill (UNC-CH) according to protocols approved by the Institutional Animal Care and Use Committee.

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Honeycutt J.B., Thayer W.O., Baker C.E., Ribeiro R.M., Lada S.M., Cao Y., Cleary R.A., Hudgens M.G., Richman D.D, & Garcia J.V. (2017). HIV persistence in tissue macrophages of humanized myeloid only mice during antiretroviral therapy. Nature medicine, 23(5), 638-643.

Publication 2017

NOD.Cg-Prkdcscid Il2rgtm1Wjl/SzJ (NSG, CB17-Prkdcscid/J mice (NOD/SCID,

Animals Capsule Cord blood Hematopoietic stem cells Human thymus Institutional animal care and use committee Kidney Laboratory animal Liver Medicine Mice Nod mice Scid Specific pathogen free Stem cell transplantation Tissue

Corresponding Organization :

Other organizations : University of North Carolina at Chapel Hill, Los Alamos National Laboratory, VA San Diego Healthcare System

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Variable analysis

independent variables

Creation of BLT mice by implanting human thymus and liver tissue under the kidney capsule of sub-lethally irradiated NSG mice
Creation of humanized MoM by transplanting sub-lethally irradiated NOD/SCID mice with cord blood or liver-derived CD34+ hematopoietic stem cells

dependent variables

Not explicitly mentioned

control variables

Irradiation of mice prior to stem cell transplantation (BLT mice: 200 rad, MoM: 250 rad)
Maintaining mice in a specific pathogen-free facility by the Division of Laboratory Animal Medicine at the University of North Carolina at Chapel Hill (UNC-CH) according to protocols approved by the Institutional Animal Care and Use Committee

controls

Positive control: Not explicitly mentioned
Negative control: Not explicitly mentioned

Annotations

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