Aortic Aneurysm Induction in Mice

In nine-week-old C57BL/6J male mice (Jackson Laboratory), hypertension was induced by angiotensin-II (1000 ng/kg/min)14 (link) or DOCA-salt treatment.14 (link), 15 (link) BAPN (150 mg/kg/day), a lysyl oxidase inhibitor, was administered for the first two weeks through a subcutaneously implanted osmotic-pump (Alzet, Durect Corp) to induce degeneration of elastic laminas. Mice were sacrificed six weeks after the surgery. Aneurysms were defined as a localized dilation of aorta greater than 50% of its adjacent intact portion of aorta.16 (link) One group of mice received an anti-hypertensive agent, amlodipine (5 mg/kg/day) in addition to angiotensin-II and BAPN. Additional mice received captopril (angiotensin-converting enzyme inhibitor, 6 mg/kg/day15 (link)) in addition to DOCA-salt treatment and BAPN.

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Kanematsu Y., Kanematsu M., Kurihara C., Tsou T.L., Nuki Y., Liang E.I., Makino H, & Hashimoto T. (2010). Pharmacologically-induced thoracic and abdominal aortic aneurysms in mice. Hypertension, 55(5), 1267-1274.

Publication 2010

Amlodipine Aneurysms Angiotensin converting enzyme inhibitor Angiotensin ii Anti hypertensive agent Aorta Bapn C57bl mice Captopril Dilation Doca salt Hypertension Lysyl oxidase Male Mice Osmotic Surgery

Corresponding Organization :

Other organizations : Tokushima University, University of California, San Francisco

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Variable analysis

independent variables

Angiotensin-II (1000 ng/kg/min) treatment
DOCA-salt treatment
BAPN (150 mg/kg/day) administration
Amlodipine (5 mg/kg/day) treatment
Captopril (6 mg/kg/day) treatment

dependent variables

Aneurysm development (defined as a localized dilation of aorta greater than 50% of its adjacent intact portion of aorta)

control variables

Nine-week-old C57BL/6J male mice (Jackson Laboratory)

positive controls

Angiotensin-II (1000 ng/kg/min) treatment
DOCA-salt treatment
BAPN (150 mg/kg/day) administration

negative controls

Amlodipine (5 mg/kg/day) treatment
Captopril (6 mg/kg/day) treatment

Annotations

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