Spindle angle determination was performed as previously described (Finegan et al, 2018 (link)). Angles were determined by drawing a first line connecting either the two spindle poles (if applicable) or along the spindle and a second line along the apical surface of the tissue, then measuring the angle between them. Spindle angle values for control w1118 are reused between Figs 1D , 3F , 4C , 5C and 6F , and for pinsp62/pinsp62 between Figs 3F , 4C and 5C .
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Spindle Angle Determination Protocol
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Other organizations : University of Rochester, University of Rochester Medical Center
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Variable analysis
independent variables
- Spindle angle determination method
- Spindle angle values
- Control w1118 genotype
- Pins^p62/pins^p62 genotype
- Control w1118 is used as a positive control across Figs 1D, 3F, 4C, 5C and 6F.
- Pins^p62/pins^p62 is used as a control across Figs 3F, 4C and 5C.
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