Bifidobacterium breve lw01 was isolated by our group previously,33 (link) and was routinely cultured in MRS Broth (BD) plus Raffinose (Amresco) supplement and 0.5% L-cysteine·HCl (Sigma-Aldrich), under anaerobic environment at 37°C. Lactobacillus rhamnosus CGMCC 1.3724 was purchased from the China General Microbiological Culture Collection Center (CGMCC), and was routinely cultured in MRS Broth (BD) plus Raffinose (Amresco) supplement under anaerobic environment at 37°C. Escherichia coli MG1655 strain, purchased from Wuhan Miaoling Bioscience & Technology Co. Ltd, was routinely cultured in Luria-Bertan Broth at 37 °C with vigorous shaking.
Protocol detail
Culturing Probiotic and Bacterial Strains
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Bifidobacterium breve
Escherichia coli
L cysteine hcl
Lactobacillus rhamnosus
Raffinose
Strain
Supplement
Corresponding Organization : Peking University
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Variable analysis
independent variables
- Culture medium of Bifidobacterium breve lw01 (MRS Broth (BD) plus Raffinose (Amresco) supplement and 0.5% L-cysteine·HCl)
- Culture medium of Lactobacillus rhamnosus CGMCC 1.3724 (MRS Broth (BD) plus Raffinose (Amresco) supplement)
- Culture medium of Escherichia coli MG1655 (Luria-Bertan Broth)
- Growth and culturing of Bifidobacterium breve lw01
- Growth and culturing of Lactobacillus rhamnosus CGMCC 1.3724
- Growth and culturing of Escherichia coli MG1655
- Anaerobic environment at 37°C for Bifidobacterium breve lw01 and Lactobacillus rhamnosus CGMCC 1.3724
- Temperature of 37°C with vigorous shaking for Escherichia coli MG1655
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