Immunodetection of Apoptosis-Related Proteins

β-lactoglobulin B (βLG) from bovine milk (18.2 kDa) (#L8005) and bovine serum albumin (BSA) (66.5 kDa) (#A7906) were purchased from MilliporeSigma, and used as negative control proteins. Rabbit anti-amyloid-β (#8243), mouse-anti-amyloid precursor protein (APP) (#2452), goat anti-rabbit IgG-HRP-linked (#7074) and horse anti-mouse IgG-HRP-linked (#7076) antibodies were obtained from Cell Signaling Technology. Affinity purified anti-FAIM antibody was obtained from rabbits immunized with CYIKAVSSRKRKEGIIHTLI peptide (located near the C-terminal region of FAIM) as previously described (Kaku and Rothstein, 2009a (link),b (link)).

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Kaku H., Ludlow A.V., Gutknecht M.F, & Rothstein T.L. (2021). Fas Apoptosis Inhibitory Molecule Blocks and Dissolves Pathological Amyloid-β Species. Frontiers in Molecular Neuroscience, 14, 750578.

Publication 2021

bovine serum albumin (BSA) horse anti-mouse IgG-HRP-linked

Amyloid Amyloid precursor protein Anti antibody Anti igg Antibodies Bovine Bovine serum albumin Goat Horse Milk Mouse Peptide Proteins Rabbit Rabbits β lactoglobulin b

Corresponding Organization : Stryker (United States)

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Variable analysis

independent variables

Rabbit anti-amyloid-β
Mouse-anti-amyloid precursor protein (APP)
Goat anti-rabbit IgG-HRP-linked
Horse anti-mouse IgG-HRP-linked
Affinity purified anti-FAIM antibody

dependent variables

Not explicitly mentioned

control variables

β-lactoglobulin B (βLG) from bovine milk (18.2 kDa)
Bovine serum albumin (BSA) (66.5 kDa)

controls

Negative controls: β-lactoglobulin B (βLG) from bovine milk (18.2 kDa) and bovine serum albumin (BSA) (66.5 kDa)

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