Growth Factor Quantification in Plasma

The concentrations of both GFs in both plasma fractions (PPP and PRP) were determined using an ELISA kit of development with antibodies to human (Human TGF-beta1 DuoSet ELISA de R&D Systems DY240-05 and Human PDGF-BB DuoSet ELISA de R&D Systems DY220, respectively), following the methodology previously published by Miguel-Pastor et al. (33 (link)). To the knowledge of the authors, there are no commercial kits for GF determination in cats, so human kits were used for GFs determination as described by other researchers (27 (link), 51 ).

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Miguel-Pastor L., Satué K., Chicharro D., Peláez P., Torres-Torrillas M., Carrillo J.M., Cerón J.J., Sopena J.J, & Rubio M. (2023). Evaluation of Platelet-Rich Plasma by means of PRGF®-Endoret® protocol in leukemia cats: PDGF-BB and TGF-ß1 valuation. Frontiers in Veterinary Science, 10, 1110055.

Publication 2023

Human TGF-beta1 DuoSet ELISA Human PDGF-BB DuoSet ELISA

Antibodies Cats Elisa Human Pastor Pdgf bb Plasma Tgf beta1

Corresponding Organization :

Other organizations : Universidad Cardenal Herrera CEU, Universidad de Murcia

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Variable analysis

independent variables

Concentrations of TGF-beta1 and PDGF-BB in plasma fractions (PPP and PRP)

dependent variables

Concentrations of TGF-beta1 and PDGF-BB in plasma fractions (PPP and PRP)

control variables

Use of human ELISA kits to determine GF concentrations in cat samples, as described by other researchers

controls

No positive or negative controls were explicitly mentioned in the provided information.

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