Synthesis and Functionalization of Spherical Gold Nanoparticles

Spherical GNPs (20nm) were synthesized using sodium citrate as a reducing agent, based on a well-established procedure 54 (link), 56 (link). GNPs were conjugated to MDDA linker (12-mercaptododecanoic acid, Sigma) then coated with D-(þ)-glucosamine hydrochloride (Sigma), due to its stability and high cell-uptake rate.

Partial Protocol Preview
This section provides a glimpse into the protocol.
The remaining content is hidden due to licensing restrictions, but the full text is available at the following link: Access Free Full Text.

Shwartz A., Betzer O., Kronfeld N., Kazimirsky G., Cazacu S., Finniss S., Lee H.K., Motiei M., Dagan S.Y., Popovtzer R., Brodie C, & Yadid G. (2017). Therapeutic Effect of Astroglia-like Mesenchymal Stem Cells Expressing Glutamate Transporter in a Genetic Rat Model of Depression. Theranostics, 7(10), 2690-2703.

Publication 2017

12-mercaptododecanoic acid

Acid Cell Glucosamine Reducing agent Sodium citrate

Corresponding Organization : Bar-Ilan University

Other organizations : Henry Ford Hospital

Top 5 similar protocols

Variable analysis

independent variables

Synthesis method: Sodium citrate as reducing agent
Conjugation of GNPs to MDDA linker
Coating of GNPs with D-(+)-glucosamine hydrochloride

dependent variables

Size of synthesized GNPs (20nm)
Cell uptake rate of GNP-glucosamine conjugates

control variables

Well-established procedure for GNP synthesis (references 54 and 56)

controls

Positive control: Likely the established procedure for GNP synthesis using sodium citrate
Negative control: Not explicitly mentioned

Annotations

Based on most similar protocols

Etiam vel ipsum. Morbi facilisis vestibulum nisl. Praesent cursus laoreet felis. Integer adipiscing pretium orci. Nulla facilisi. Quisque posuere bibendum purus. Nulla quam mauris, cursus eget, convallis ac, molestie non, enim. Aliquam congue. Quisque sagittis nonummy sapien. Proin molestie sem vitae urna. Maecenas lorem.

As authors may omit details in methods from publication, our AI will look for missing critical information across the 5 most similar protocols.

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!