Adenoviral Transduction of Cardiomyocytes

Recombinant adenovirus of p16^INK4a (Ad5:cTNT-INK4a), p16^INK4a scrambled shRNA (Ad5:cTNT-INK4a RNAi), and adenovirus of control (Ad5:cTNT-CON) were designed by CMs specific cTNT promoter obtained from GeneChem Company (China). Ad5:cTNT-CON served as an empty control virus for Ad5:cTNT-INK4a and Ad5:cTNT-INK4a RNAi. It was found in previous studies of primary CMs in vitro that we used a multiplicity of infection (MOI) = 50 as a viral titer to transfect CMs 48 hours after performing other relevant experimental assays [17 (link), 21 (link)]. In vivo, 1-day-old neonatal mice underwent AR + intramyocardial injection: Ad5:cTNT-INK4a RNAi (3.0 × 10⁷ PFU/mouse, total volume = 6 μl/mouse) or Ad5-cTNT-CON (3.0 × 10⁷ PFU/mouse, total volume = 6 μl/mouse).

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Sun J., Zhou L., Yang T., Deng B., Bao Y., Gu L., Wang H, & Wang L. (2023). P16INK4a Regulates ROS-Related Autophagy and CDK4/6-Mediated Proliferation: A New Target of Myocardial Regeneration Therapy. Oxidative Medicine and Cellular Longevity, 2023, 1696190.

Publication 2023

Adenovirus Assays Infection Ink4a Mouse Neonatal P16ink4a Rnai Shrna Virus

Corresponding Organization : Jiangsu Province Hospital

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Variable analysis

independent variables

Ad5:cTNT-INK4a (recombinant adenovirus of p16^{INK4a})
Ad5:cTNT-INK4a RNAi (p16^{INK4a} scrambled shRNA)
Ad5:cTNT-CON (adenovirus of control)

dependent variables

Unspecified

control variables

Multiplicity of infection (MOI) = 50 used as viral titer to transfect cardiomyocytes (CMs) in vitro
Neonatal mice used for in vivo experiments
Intramyocardial injection volume = 6 μl/mouse

controls

Positive control: Ad5:cTNT-CON (empty control virus for Ad5:cTNT-INK4a and Ad5:cTNT-INK4a RNAi)

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