The MCF-7 cell growth inhibition assay was carried out as described previously45 (link). A broth micro-dilution method was used to determine the growth inhibition IC50 values. Compounds were half-log serial diluted using cell culture media into 96-well TC-treated round bottom plates (Corning Inc., Corning, NY). Cells were plated at a density of 5000 cells/well and then incubated under the same culture conditions for 2 days at which time an MTT ((3-(4,5-dimethylthi-azole-2-yl)-2,5-diphenyltetrazolium bromide) cell proliferation assay (ATCC, Manassas, VA) was performed to obtain dose response curves.
Protocol detail
MCF-7 Cell Growth Inhibition Assay
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Assay
Azole
Bromide
Cell culture
Cell proliferation
Cells
Culture media
Dilution method
Inhibition
Mcf 7 cell
Corresponding Organization :
Other organizations : University of Illinois Urbana-Champaign, Colorado State University, École Polytechnique Fédérale de Lausanne, Institut Pasteur Korea, Chinese Academy of Sciences
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Variable analysis
independent variables
- Compounds
- Growth inhibition IC50 values
- Cell culture media
- Cell density (5000 cells/well)
- Incubation time (2 days)
- MTT cell proliferation assay
- Not explicitly mentioned
- Not explicitly mentioned
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