A total of 3×105 cells were seeded in six-well plates and incubated overnight. After treatment with 100 ng/ml of rhL1CAM or 100 nM of siL1CAM for 24 h, cells were incubated with 5% CO2 at 37°C for 24, 48 and 72 h. Viable cells were stained with 0.4% trypan blue solution (Gibco; Thermo Fisher Scientific, Inc.) at RT for 5 min and counted using a hemocytometer at each time-point.
Protocol detail
Cell Viability Assay with L1CAM
Partial Protocol Preview
This section provides a glimpse into the protocol.
The remaining content is hidden due to licensing restrictions, but the full text is available at the following link:
Access Free Full Text.
Corresponding Organization :
Other organizations : Seoul National University
Top 5 similar protocols
Variable analysis
independent variables
- Treatment with 100 ng/ml of rhL1CAM
- Treatment with 100 nM of siL1CAM
- Viable cell count
- Total number of cells seeded (3x10^5)
- Incubation conditions (5% CO2 at 37°C)
- Incubation duration (24, 48, and 72 hours)
- Trypan blue staining and counting using a hemocytometer
Annotations
Based on most similar protocols
Etiam vel ipsum. Morbi facilisis vestibulum nisl. Praesent cursus laoreet felis. Integer adipiscing pretium orci. Nulla facilisi. Quisque posuere bibendum purus. Nulla quam mauris, cursus eget, convallis ac, molestie non, enim. Aliquam congue. Quisque sagittis nonummy sapien. Proin molestie sem vitae urna. Maecenas lorem.
Sign up for free or login to display all annotations
As authors may omit details in methods from publication, our AI will look for missing critical information across the 5 most similar protocols.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!