To assess the effect of different oxidative stressors and chemicals on the cell-based reporters S2 cells were transiently transfected with the reporter plasmids by the calcium phosphate method. 8 hours after the PBS wash and medium change, the transfected cells were transferred to 96-well plates and treated with 25 µM oltipraz, 100 µM DEM, 100 µM NaAsO2 (J.T.Baker (Phillipsburg, NJ)) and were incubated at 25°C for 24 hrs.
Oxidative Stress Regulation of Reporter Genes
To assess the effect of different oxidative stressors and chemicals on the cell-based reporters S2 cells were transiently transfected with the reporter plasmids by the calcium phosphate method. 8 hours after the PBS wash and medium change, the transfected cells were transferred to 96-well plates and treated with 25 µM oltipraz, 100 µM DEM, 100 µM NaAsO2 (J.T.Baker (Phillipsburg, NJ)) and were incubated at 25°C for 24 hrs.
Corresponding Organization :
Other organizations : University of Rochester Medical Center
Protocol cited in 17 other protocols
Variable analysis
- Different oxidative stressors (Paraquat, oltipraz, DEM, NaAsO2)
- Treatment of reporter flies with 1 mM oltipraz for 48 hours
- Reporter gene expression in transgenic flies
- Reporter gene expression in S2 cells
- Age of flies (1 week old)
- Flies were mated for one day and then separated into males and females
- Flies were starved for 2 hours before treatment
- Concentration of sucrose solution (5%)
- Incubation temperature for S2 cells (25°C)
- Incubation time for S2 cells (24 hours)
- None specified
- None specified
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