Characterization of Osteosarcoma Cell Lines

Human OS cells (HOS, MG-63, N-methyl-N-nitro-N-nitrosoguanidine (MNNG)/HOS and 143B) and human umbilical vein endothelial cells (HUVEC) were purchased from the American Type Culture Collection (ATCC, Manassas, VA, USA). All cell lines were authenticated by DNA short tandem repeat profiling and experiments were conducted within 6 months of resuscitation. OS cells were maintained in DMEM medium containing 10% FBS (Atlanta Biologicals, Lawrenceville, GA, USA), 100 U/ml penicillin and 100 μg/ml streptomycin (Invitrogen, Carlsbad, CA, USA), while HUVECs were maintained in 200PRF medium (Invitrogen) supplemented with low serum growth supplement (Invitrogen). All cell cultures were maintained at 37°C with 5% CO₂. 143B cells were serum starved overnight and treated with KN-93 (10 μM) and/or CBO-P11 (1μM) (Millipore) for 24 hours [14 (link)].