Synthesis and Characterization of AαC Metabolites

AαC was purchased from the Toronto Research Chemicals (Toronto, ON, Canada). [4b,5,6,7,8,8a-¹³C₆]AαC was a gift from Dr. Daniel Doerge, National Center for Toxicological Research (Jefferson, AR). Human liver microsomes were obtained from the Tennessee Donor Services, Nashville, TN, and kindly provided by Prof. F. P. Guengerich, Vanderbilt University. DMSO, ethoxyresorufin, methoxyresorufin, methanol, acetonitrile, ascorbic acid, hydrochloric acid, ammonium acetate, sulfatase from Helix pomatia (≥10,000 units/g solid) and β-Glucuronidase from Helix pomatia (≥30,000 units/g solid) were purchased from Sigma Aldrich (St. Louis, MO, USA). AαC-3-OH and AαC-6-OH were prepared with human liver microsomes or rat liver microsomes and spectroscopically characterized as previously reported.^{17 (link),34 (link)}N-(deoxyguanosin-8-yl)-2-amino-9H-pyrido[2,3-b]indole (dG-C8-AαC) and [¹³C₁₀]-dG-C8-AαC were prepared as previously described.^{27 (link)}