Evaluating Pseudomonas Infection in CF Cells

IB3-1 cells, an adeno-associated virus-transformed human bronchial epithelial cell line derived from a CF patient (ΔF508/W1282X) and obtained from LGC Promochem, were grown as described previously [39 (link)]. Cells were infected with P. aeruginosa strains at a multiplicity of infection (MOI) of 100. Cell viability was evaluated using the CellTiter 96^® Non-Radioactive Cell Proliferation Assay (MTT) kit (Promega, G4000), according to manufacturer’s instructions.

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Ferrara S., Falcone M., Macchi R., Bragonzi A., Girelli D., Cariani L., Cigana C, & Bertoni G. (2017). The PAPI-1 pathogenicity island-encoded small RNA PesA influences Pseudomonas aeruginosa virulence and modulates pyocin S3 production. PLoS ONE, 12(6), e0180386.

Publication 2017

CellTiter 96® Non-Radioactive Cell Proliferation Assay (MTT) kit

Adeno associated virus Aeruginosa p Assay Bronchial Cell proliferation Cell viability Cells Human Infection Patient Promega Radioactive Strains Transformed cell line

Corresponding Organization : Fondazione IRCCS Ca' Granda Ospedale Maggiore Policlinico

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Variable analysis

independent variables

P. aeruginosa strains

dependent variables

Cell viability

control variables

IB3-1 cells, an adeno-associated virus-transformed human bronchial epithelial cell line derived from a CF patient (ΔF508/W1282X) and obtained from LGC Promochem
Multiplicity of infection (MOI) of 100

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