The HBV DNA concentration in the HBV-HCC tissue was quantified as copies per microgram of genomic DNA with ABI 7300 TaqMan platform (Life Technologies, Carlsbad, CA, USA) by real-time PCR. HBV genotypes were determined by multiplex-PCR as described previously.18 (link) The DNA sequences flanking the small S region were amplified with the primer pairs listed in Table 1 according to the NCBI database (http://www.ncbi.nlm.nih.gov/genome/5536 ). Cyclic sequencing was performed with a BigDye Terminator v3.1 Cycle Sequencing Kit (Life Technologies) by ABI PRISM 3100 Genetic Analyzer (Life Technologies). Mutations were confirmed by repeating the analysis on both the strands.
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HBV Genotyping and Quantification in HCC Tissue
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Corresponding Organization :
Other organizations : Eastern Hepatobiliary Surgery Hospital, Second Military Medical University, Hebei Medical University, Fourth Hospital of Hebei Medical University
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Variable analysis
independent variables
- HBV genotypes
- HBV DNA concentration in HBV-HCC tissue
- Genomic DNA as reference for HBV DNA quantification
- Primers listed in Table 1 for DNA sequence amplification
- Positive control: None mentioned
- Negative control: None mentioned
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