Candida albicans-Induced Cytotoxicity Assay

Damage induction by C. albicans in TR146 cells was performed as described [70 (link)]. Briefly, monolayers of TR146 cells in 96-well tissue culture plates prepared as described above were infected with2 x 10⁴ yeast cells per well and incubated for 24 hours at 37°C and 5% CO₂. Control wells were incubated with medium only or with 1% Triton-X-100 to determine 100% damage. LDH release into the supernatant was quantified with the LDH cytotoxicity kit (Roche) according to the manufacturer’s instructions. For assessing LDH concentrations in the supernatant of reconstructed human epidermis, a standard curve was generated with recombinant LDH enzyme.