Evaluating MYB and NF-κB Expression in Skin Tumors
MYB overexpression in cylindromas has been reported in several earlier studies57 (link). We attempted to assess the MYB expression status of 26 samples (11 cylindromas, 6 spiradenomas, and 9 high-grade spiradenocarcinomas) using immunohistochemistry (IHC) (Supplementary Data 4). IHC was performed on 4-μm-thick formalin-fixed paraffin-embedded whole-tissue sections following antigen retrieval with Target Retrieval solution (pH 6.1; Dako, Carpinteria, CA, USA) in a pressure cooker using a rabbit monoclonal anti-MYB monoclonal antibody (1:200 dilution; clone EP769Y; Abcam, Cambridge, MA, USA) and the Envision + polymer detection system (Dako). Immunohistochemistry for p65 was performed using an anti-NF-κB p65 antibody (1:5000 dilution; clone D14E12; Cell Signaling Technology, Danvers, MA, USA) as described for MYB above.
Rashid M., van der Horst M., Mentzel T., Butera F., Ferreira I., Pance A., Rütten A., Luzar B., Marusic Z., de Saint Aubain N., Ko J.S., Billings S.D., Chen S., Abi Daoud M., Hewinson J., Louzada S., Harms P.W., Cerretelli G., Robles-Espinoza C.D., Patel R.M., van der Weyden L., Bakal C., Hornick J.L., Arends M.J., Brenn T, & Adams D.J. (2019). ALPK1 hotspot mutation as a driver of human spiradenoma and spiradenocarcinoma. Nature Communications, 10, 2213.
Other organizations :
Wellcome Sanger Institute, Maasstad Ziekenhuis, Dermatopathologie Friedrichshafen, Institute of Cancer Research, University of Ljubljana, University Hospital Centre Zagreb, Institut Jules Bordet, Cleveland Clinic, University of Calgary, University of Michigan–Ann Arbor, Edinburgh Cancer Research, Universidad Nacional Autónoma de México, Brigham and Women's Hospital, Harvard University
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