Proteasome and Cytoskeletal Dynamics

We used rat anti-mouse LAMP1 (BD Bioscience, #553792, 1:200), rabbit anti-mouse α-Tubulin (Abcam, ab#6160, 1:200), rabbit anti-acetyl-α-Tubulin (Lys40; cell signaling, #5335, 1:200), rabbit anti-mouse γ-Tubulin (Abcam, #Ab11317, 1:1000), rabbit anti-mouse S4/19S RP (Abcam, #Ab223765, 1:100), rabbit anti-mouse αβ/20S proteasome (Abcam, #Ab22673, 1:200), anti-mouse Ecm29 (Abcam, #Ab28666, 1:100), mouse anti-mouse Ubiquitin P4D1 (Santa Cruz, #Sc-8017, 1:1000), anti-mouse anti-actin (cloneC4, ImmunO, #691001), rabbit anti-mouse Arp2 (Cellsignal, #5614, 1:500), goat anti-mouse IgGFab² (Jackson ImmunoResearch), rabbit anti-OVA (Sigma-Aldrich, #C6534, 1:500). For secondary antibodies: donkey anti-rabbit IgG-Alexa488 (LifeTech, 1:200), goat anti-rabbit IgG-Alexa546 (ThermoScientific, 1:200), Donkey anti-rat IgG-Alexa546 (ThermoScientific, 1:200), Donkey anti-rat-Alexa647 (ThermoScientific, 1:200), Phalloidin-Alexa-647 (Life Technology, #22287, 1:200), DAPI (Abcam). Ovalbumin and Nocodazole were purchased from Sigma-Aldrich, MG-132, and Epoxomicin were purchased from Merk (Millipore). Bsc2118-FL-Bodipy was kindly provided by Ulrike Kuckelkorn (Mlynarczuk-Bialy et al., 2014 (link)).