SSTR2-Expressing Cell Line Panel

H1299-7 cells, constitutively overexpressing SSTR2 (a gift from Buck Rogers, Mallinckrodt Institute, St Louis, Missouri), AR42J cells expressing high SSTR2 and SKNBE-2 expressing low levels of SSTR2, are as described in Cullinane et al19 (link). The identity of the H1299-7 and SKNBE-2 cell lines was confirmed through STR profiling (AGRF, Melbourne, Australia). Nedisertib and AZD-7648 were purchased from Med Chem Express. LuTate was produced using [DOTA⁰, Tyr³] octreotate and carrier-added [¹⁷⁷Lu] Lutetium chloride (IDB, the Netherlands).

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Waldeck K., Van Zuylekom J., Cullinane C., Gulati T., Simpson K.J., Tothill R.W., Blyth B, & Hicks R.J. (2023). A genome-wide CRISPR/Cas9 screen identifies DNA-PK as a sensitiser to 177Lutetium-DOTA-octreotate radionuclide therapy. Theranostics, 13(14), 4745-4761.

Publication 2023

Nedisertib AZD-7648

177lu Azd 7648 Cell lines Cells Chloride Lutetium Nedisertib Sstr2

Corresponding Organization : University of Melbourne

Other organizations : St Vincent's Hospital

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Variable analysis

independent variables

SSTR2 overexpression in H1299-7 cells
SSTR2 expression levels in AR42J and SKNBE-2 cell lines
Nedisertib treatment
AZD-7648 treatment
LuTate treatment

dependent variables

Not explicitly mentioned

control variables

STR profiling to confirm the identity of H1299-7 and SKNBE-2 cell lines
Use of carrier-added [177Lu] Lutetium chloride for LuTate production

controls

Positive control: AR42J cells expressing high SSTR2
Negative control: SKNBE-2 cells expressing low levels of SSTR2

Annotations

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