DNA was extracted from the buccal swab samples using the QIAamp DNA Blood Mini Kit (QIAGEN, Hilden, Germany) according to the manufacturer’s instructions.
Genotyping of CYP3A4 (−392 G>A, rs2740574), CYP3A5 (6986A>G, rs776746), CYP2C9 (430 C>T, rs1799853, and 1075A>T, rs1057910), and CYP2C19 (681 G>A, rs4244285 and −806 C>T, rs12248560) was performed by TaqMan® Drug Metabolism Genotyping Assays (Thermo Fisher Scientific, USA) on ViiA Real-Time PCR System (Applied Biosystems™, Foster City, CA, USA) according to manufacturer’s protocol.
CYP1A1 (1759A>G, rs4646903) genotypes were analyzed by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP), using the MspI restriction enzyme (New England Biolabs, Hitchin, United Kingdom), as previously described (Feldman et al., 2009 (link)).
Genotyping of CYP3A4 (−392 G>A, rs2740574), CYP3A5 (6986A>G, rs776746), CYP2C9 (430 C>T, rs1799853, and 1075A>T, rs1057910), and CYP2C19 (681 G>A, rs4244285 and −806 C>T, rs12248560) was performed by TaqMan® Drug Metabolism Genotyping Assays (Thermo Fisher Scientific, USA) on ViiA Real-Time PCR System (Applied Biosystems™, Foster City, CA, USA) according to manufacturer’s protocol.
CYP1A1 (1759A>G, rs4646903) genotypes were analyzed by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP), using the MspI restriction enzyme (New England Biolabs, Hitchin, United Kingdom), as previously described (Feldman et al., 2009 (link)).
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