Quantitative RT-PCR Protocol for Gene Expression

Quantitative RT-PCR was performed as described (Tisch et al., 2011a (link)). All reactions were performed on a CFX96 Real-Time system machine (Bio-Rad) with the GoTaq qPCR Master Mix (Promega, Madison, WI, USA) and primers for cbh1 and L6e (reference gene) (Tisch et al., 2011a (link)). At least two biological replicates were analyzed with three technical replicates. Data was analyzed with qbase+ (Biogazelle) and the CFX Maestro (Bio-Rad) software (statistics including ANOVA).

Free full text: Click here

Stappler E., Walton J.D., Beier S, & Schmoll M. (2017). Abundance of Secreted Proteins of Trichoderma reesei Is Regulated by Light of Different Intensities. Frontiers in Microbiology, 8, 2586.

Publication 2017

CFX96 Real-Time system machine GoTaq qPCR Master Mix qbase+ CFX Maestro

Anova Biological Gene Primers Promega Rt pcr Tisch

Corresponding Organization : Austrian Institute of Technology

Other organizations : Michigan State University

Top 5 similar protocols

Variable analysis

independent variables

None explicitly mentioned

dependent variables

MRNA expression levels of cbh1

control variables

Quantitative RT-PCR protocol (Tisch et al., 2011a)
Use of CFX96 Real-Time system machine (Bio-Rad)
Use of GoTaq qPCR Master Mix (Promega)
Use of primers for cbh1 and L6e (reference gene) (Tisch et al., 2011a)
At least two biological replicates analyzed
Three technical replicates performed

controls

Positive control: Not explicitly mentioned
Negative control: Not explicitly mentioned

Annotations

Based on most similar protocols

Etiam vel ipsum. Morbi facilisis vestibulum nisl. Praesent cursus laoreet felis. Integer adipiscing pretium orci. Nulla facilisi. Quisque posuere bibendum purus. Nulla quam mauris, cursus eget, convallis ac, molestie non, enim. Aliquam congue. Quisque sagittis nonummy sapien. Proin molestie sem vitae urna. Maecenas lorem.

As authors may omit details in methods from publication, our AI will look for missing critical information across the 5 most similar protocols.

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!