For the samples from Populous collection, procedures concerning DNA extraction and sample processing have previously been described [48 (link),49 (link)].
For the group of psoriatic patients, genomic DNA was extracted from whole blood using the MagNa Pure LC 2.0 (Roche, Basel, Switzerland). NanoDrop 1000 (Thermo Fisher Scientific Inc., Waltham, MA, USA) and Qubit 2.0 (Thermo Fisher Scientific Inc., Waltham, MA, USA) were used to determine DNA quality and concentrations. Afterward, DNA samples underwent PCR reaction for sex verification [50 ].
For the group of psoriatic patients, genomic DNA was extracted from whole blood using the MagNa Pure LC 2.0 (Roche, Basel, Switzerland). NanoDrop 1000 (Thermo Fisher Scientific Inc., Waltham, MA, USA) and Qubit 2.0 (Thermo Fisher Scientific Inc., Waltham, MA, USA) were used to determine DNA quality and concentrations. Afterward, DNA samples underwent PCR reaction for sex verification [50 ].
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