Construction of Isogenic K. pneumoniae Mutants

K. pneumoniae NTUH-K2044 (a strain that resulted in pyogenic liver abscess in a 66 year old patient) was kindly provided by Dr. Jin Town Wang of the National Taiwan University Hospital, Taipei, Taiwan [10] (link). E. coli SM10 lambda pir and pUT-Km was used to create isogenic mutants. Genomic DNA, plasmid, restriction digestion, DNA elution (Qiagen), ligation, transformation, conjugation, DNA sequencing (Applied Biosystems) were performed as previously described [22] (link), [23] (link), [24] . Primers used in the present study were custom-synthesized (Eurofins MWG operons, Germany).

Free full text: Click here

Srinivasan V.B., Singh B.B., Priyadarshi N., Chauhan N.K, & Rajamohan G. (2014). Role of Novel Multidrug Efflux Pump Involved in Drug Resistance in Klebsiella pneumoniae. PLoS ONE, 9(5), e96288.

Publication 2014

DNA sequencing

Digestion E coli Genomic K pneumoniae Ligation Operons Patient Plasmid Primers Pyogenic liver abscess Strain

Corresponding Organization :

Other organizations : Institute of Microbial Technology

Top 5 similar protocols

Variable analysis

independent variables

Creation of isogenic mutants using E. coli SM10 lambda pir and pUT-Km

dependent variables

Not explicitly mentioned

control variables

Genomic DNA extraction, plasmid extraction, restriction digestion, DNA elution (Qiagen), ligation, transformation, conjugation, DNA sequencing (Applied Biosystems) were performed as previously described in [22], [23], [24]

positive controls

None mentioned

negative controls

None mentioned

Annotations

Based on most similar protocols

Etiam vel ipsum. Morbi facilisis vestibulum nisl. Praesent cursus laoreet felis. Integer adipiscing pretium orci. Nulla facilisi. Quisque posuere bibendum purus. Nulla quam mauris, cursus eget, convallis ac, molestie non, enim. Aliquam congue. Quisque sagittis nonummy sapien. Proin molestie sem vitae urna. Maecenas lorem.

As authors may omit details in methods from publication, our AI will look for missing critical information across the 5 most similar protocols.

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!