Protocol detail

Comprehensive Western Blotting Protocol

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Standard protocols were followed for Western blotting assays [12 (link)]. The primary antibodies used were LAMP1, Tubulin, Dynamin, Clathrin, Caveolae, Na-K ATPase (Cell Signaling Technology, Boston, MA, USA), IL-20R1, IL-20R2, IL-22R1 (Abcam), GRP78 (Santacruz Biotechnology), and MDA-7/IL-24 (Genhunter, Nashville, TN, USA). Secondary antibodies used in this study were from Cell Signaling Technology.

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Pradhan A.K., Bhoopathi P., Talukdar S., Das S.K., Emdad L., Sarkar D., Ivanov A.I, & Fisher P.B. (2019). Mechanism of internalization of MDA-7/IL-24 protein and its cognate receptors following ligand-receptor docking. Oncotarget, 10(49), 5103-5117.

Publication 2019

LAMP1 Tubulin Clathrin IL-22R1 GRP78

Antibodies Caveolae Clathrin Dynamin Grp78 Il 22r1 Il 24 Lamp1 Na k atpase Tubulin Western blotting assays

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Variable analysis

independent variables

Primary antibodies: LAMP1, Tubulin, Dynamin, Clathrin, Caveolae, Na-K ATPase (Cell Signaling Technology, Boston, MA, USA), IL-20R1, IL-20R2, IL-22R1 (Abcam), GRP78 (Santacruz Biotechnology), and MDA-7/IL-24 (Genhunter, Nashville, TN, USA)

dependent variables

Measured outcomes from Western blotting assays

control variables

Secondary antibodies used in this study were from Cell Signaling Technology

controls

Positive controls not specified
Negative controls not specified

Annotations

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