Immunofluorescence staining was performed as described [22] (link), [24] (link), [35] (link), [37] (link), [40] (link), [52] . Briefly, cells were fixed with methanol, permeabilized with 1% NP-40, and blocked with 10% BSA, followed by incubating with CD73, CD44, CD90, CD117/c-kit, CD29, CD133, CD105/endoglin, CD166/ALCAM, or BMPR-II antibody (Santa Cruz Biotechnology) for 1 hr at room temperature. After being washed, cells were incubated with Texas Red-labeled secondary antibody (Santa Cruz Biotechnology) for 30 min. Cell nuclei were stained with DAPI. Stains were examined under a fluorescence microscope. Stains without primary antibodies, or with control IgG, were used as negative controls.
Wang N., Zhang W., Cui J., Zhang H., Chen X., Li R., Wu N., Chen X., Wen S., Zhang J., Yin L., Deng F., Liao Z., Zhang Z., Zhang Q., Yan Z., Liu W., Ye J., Deng Y., Wang Z., Qiao M., Luu H.H., Haydon R.C., Shi L.L., Liang H, & He T.C. (2014). The piggyBac Transposon-Mediated Expression of SV40 T Antigen Efficiently Immortalizes Mouse Embryonic Fibroblasts (MEFs). PLoS ONE, 9(5), e97316.
Other organizations :
Molecular Oncology (United States), University of Chicago Medical Center, Army Medical University, Southwest Hospital, First Affiliated Hospital of Chongqing Medical University, Chongqing Medical University, Binzhou University, Binzhou Medical University, University of Chicago, Xiangya Hospital Central South University, Central South University, Zhongnan Hospital of Wuhan University, Wuhan University, Chongqing University
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