DRGs were extracted from 7 day old Wistar rats and neurons were dissociated as previously described (8 (link), 19 (link)). Cultures were maintained on 10 mm glass coverslips pre-coated with poly-D-lysine and laminin and cells were left to grow for 48 hours in DMEM supplemented with GlutaMAX I (Invitrogen), 10% fetal bovine serum, penicillin (50 U/ml), and streptomycin (50 μg/ml) in a humidified incubator (5% CO2, 37°C). For transfection with the EYFP-QL or CEPIA constructs, the Lonza Nucleofector I was used and transfection was done before plating out the cells as described (70 (link)).