The Roche cell death detection kit was utilized to quantify the occurrence of apoptosis in MCF7 and HCT116 cancer cells treated with Dox (positive control) and Lip-DT-Dox. The quantification of cytoplasmic histone-associated DNA fragments was performed using an ELISA kit. The results are presented as the fold increase in the enrichment factor of cytoplasmic nucleosomes.50 (link)