Plasma Biomarker Measurement Protocol

Plasma assays were performed as previously described (31 (link), 36 (link)). Briefly, plasma glucose was measured on a Beckman Analyzer II (Beckman, Fullerton, CA, USA) in rats and on a HemoCue Glucose 201 Analyzer (HemoCue Inc., CA, USA) in mice. Plasma FFA were measured with an enzymatic colorimetric kit (Wako Industries, Neuss, Germany). Radioimmunoassays specific for rat/mouse insulin and C-peptide (Linco, St. Charles, MO, USA) were used to determine their plasma concentrations. All assays were performed in duplicate.

Free full text: Click here

Ivovic A., Yung J.H., Oprescu A.I., Vlavcheski F., Mori Y., Rahman S.M., Ye W., Eversley J.A., Wheeler M.B., Woo M., Tsiani E, & Giacca A. (2024). β-Cell Insulin Resistance Plays a Causal Role in Fat-Induced β-Cell Dysfunction In Vitro and In Vivo. Endocrinology, 165(5), bqae044.

Publication 2024

Beckman Analyzer II HemoCue Glucose 201 Analyzer

Corresponding Organization :

Other organizations : University of Toronto, Brock University, University Health Network, Toronto General Hospital, Showa University

Top 5 similar protocols

Variable analysis

independent variables

None explicitly mentioned

dependent variables

Plasma glucose
Plasma FFA
Plasma insulin
Plasma C-peptide

control variables

Assay methods (Beckman Analyzer II, HemoCue Glucose 201 Analyzer, enzymatic colorimetric kit, radioimmunoassays)
Sample type (plasma)

controls

Positive control: Not specified
Negative control: Not specified

Annotations

Based on most similar protocols

Etiam vel ipsum. Morbi facilisis vestibulum nisl. Praesent cursus laoreet felis. Integer adipiscing pretium orci. Nulla facilisi. Quisque posuere bibendum purus. Nulla quam mauris, cursus eget, convallis ac, molestie non, enim. Aliquam congue. Quisque sagittis nonummy sapien. Proin molestie sem vitae urna. Maecenas lorem.

As authors may omit details in methods from publication, our AI will look for missing critical information across the 5 most similar protocols.

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!